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I have switched from Alexa 405 to DyLight 405 coupled secondary antibodies, as it is much more stable,  even as stable as other channels (Alexa488 / 555 / 647 usually). Coupled with Prolong Gold as a mountant I barely see any photobleaching when imaging on a widefield microscope. Also on a widefield system, getting a true 405 filter cube rather than a standard DAPI one significantly enhances the signal. 

Christophe


Le 28 juin 2012 à 18:34, Kurt Thorn <[log in to unmask]> a écrit :

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> I haven't used any of these myself, but I've compiled a list of commercially 405nm excitable dyes for our users here:
> http://nic.ucsf.edu/dokuwiki/doku.php?id=dyes:405nm
> 
> If you try any it would be great to get some feedback about how they perform.
> 
> Best,
> Kurt
> 
> On 6/28/2012 3:00 AM, Graham Wright wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> Dear all,
>> 
>> I'm looking for recommendations for a blue dye (ex. 405nm) to be used on a
>> secondary antibody. Unfortunately, the other wavelengths are already taken,
>> which would normally be my suggestion.
>> 
>> I've had a look through the archives of this list and there is confirmation
>> that they are not ideal and tend to bleach easily, but are there any
>> updates on this since 2008/2010?
>> 
>> Any comments and recommendations will be appreciated about the options:
>> Alexa 405, Pacific Blue, Cascade Blue...
>> 
>> Much obliged,
>> Graham
>>