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Date: | Tue, 9 Mar 1993 12:17:48 MET |
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I have no experience as far as the confocal microscope is concerned,
but I am used to intensify DAB for conventional light microscopy.
The DAB precipitate has a structure which could be compared with
that of melanin and, indeed, procedures based on its reducing
properties work quite well:
the so-called reaction of Chevremont - Fredericq based on the
formation 'prussian blue' (ferric ferrocyanide) gives nice blue
green precipitates at DAB containing sites;
any silver reaction suitable for detection of 'argentaffin' or
'APUD' cells also works great: I usually use the Masson's ammoniacal
silver solution for this. On neurons filled with DAB labelled anti
calbindin antibody, this produces very nice Golgi-like pictures.
Beware, curiously enough, these reactions do not work on cobalt
enhanced DAB.
Hope this helps...
Jean Desclin
Associate Prof of Histology
Dpt of Histology - Fac. of Medicine
Brussels' Free University (U.L.B.)
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