At  6:14 PM 2/9/95 +0100, Anne McKinney wrote:
>Does anyone know of a fluorescent dye other than Lucifer Yellow
>which can be
>used for intracellular recording and is fixable?
>                Many thanks,
>                Anne McKinney
>
>
 
How about carboxyfluorescein? Lucifer Yellow is the best tracer
to study endocytosis for me, except using with 488nm laser.
Thus I am using carboxyfluorescein as a replacement of LY. It
may not be fixable and there observed unvigorous fluorescence
in cytosole, which might be caused by leaking before or after
fixation. But still I could see the similar structure as in the
prep of LW.
 
Also I have tried the fixable FITC-dextran of Molecular Prob.
However I could not get enough fluorescence from the fine
structure.
 
You can refere the usage of carboxyfluorecscein to;
  G.Rao et al., Journal of Neuroscience Methods, 16 (1986)
pp.251-263
 
I hope somebody post better dye, which can be used as an tracer
under 488nm observation.
 
Akira Kihara
 
PS. Please post about scientific topics to increase S/N ratio!
              _____________________________________________
             | Akira Kihara <[log in to unmask]>
             |      Laboratory of Biology, Hosei University
             |      Fujimi 2-17, Chioyoda, Tokyo 102, JAPAN
             |
             |      FAX 81-3-3264-9368, Phone 81-3-3264-9390