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Would second harmonic imaging show up processed starch though?  Polarised light shows up raw starch grains but will it work if the grains have taken up water and changed structure?  They lose the maltese cross which usually shows up in polarised light.



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Guy Cox
Sent: Sunday, 20 February 2011 12:45 a.m.
To: [log in to unmask]
Subject: Re: fluorescent staining of starch

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Valerie,



          By far the simplest approach is to use second harmonic
imaging.  Starch gives a very strong SH signal.




Guy



Optical Imaging Techniques in Cell Biology

by Guy Cox    CRC Press / Taylor & Francis

     http://www.guycox.com/optical.htm
<http://www.guycox.com/optical.htm>

______________________________________________

Associate Professor Guy Cox, MA, DPhil(Oxon)

Australian Centre for Microscopy & Microanalysis,

Madsen Building F09, University of Sydney, NSW 2006



Phone +61 2 9351 3176     Fax +61 2 9351 7682

             Mobile 0413 281 861

______________________________________________

      http://www.guycox.net <http://www.guycox.net>





From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Valerie Lawrence
Sent: Saturday, 19 February 2011 6:49 AM
To: [log in to unmask]
Subject: fluorescent staining of starch



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Hi all,
We are looking for a method of imaging starch in paper using confocal
microscopy.  None of the fluorescent dyes we've tried (basic fuchsin,
crystal
violet, SF light) are useful since they also stain the cellulose in the
wood
fibres.  Starch has been hydrolyzed.  Any suggestions would be greatly
appreciated.
Thank you.
Valerie Lawrence

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