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We are having a similar problem with eGFP/eYFP unmixing, so we would be
interested to hear any suggestions about it too.

Thanks

Hugo Caldas
Columbus Children's Hospital
Columbus, OH

> ----------
> From:         Tami Casavan
> Reply To:     Confocal Microscopy List
> Sent:         Thursday, January 22, 2004 9:46 AM
> To:   [log in to unmask]
> Subject:      Online Fingerprinting
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> We are using a Zeiss LSM 510 META to perform linear unmixing for eCFP and
> eGFP.  When unmixing a sample with ONLY eCFP using spectra for both
> fluorochromes, the result includes a low intensity grainy textured signal
> for
> eGFP.  Does anyone know a way to eliminate this erroneous signal?  This
> appears using online fingerprinting as well as linear unmixing with only
> advanced linear unmixing checked for the options.
>
> Thanks in advance for any suggestions.
>
> Tami Casavan
> UND Medical School
>
>