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a trick from the SEM world is to dissolve the adhesive from scotch 
tape with chloroform. A drop of the solution on a cover slip will 
leave a VERY thin adhesive layer that will not dissolve in water.

At 10:00 AM 2/17/2007 -0500, you wrote:
>Search the CONFOCAL archive at 
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>
>Hi all,
>
>I'm trying to stick 6 micron polystyrene beads to a glass coverslip 
>and mount them in water.  I'm drying the beads onto the coverslip, 
>but they wash away when I add the water.  We do this all the time 
>mounting them in glycerol and it works fine.  Any suggestions on how 
>to get the beads to stick?
>
>Thanks, Jennifer
>
>--
>Jennifer Waters, Ph.D.
>Director, Nikon Imaging Center at Harvard Medical School