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If you are using a cytoplasmic GFP, it usually goes into the nucleus as its
size is smaller than the exclusion limit for proteins entering the nucleus.
Nina Allen
On 11/8/03 11:49 PM, "shagufta rehman" <[log in to unmask]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> hi,
>  I am using EGFP tagged fusion proteins for my study
> which is basically an intracellular localization
> study. I am monitoring the expression of EGFP by
> confocal microscope. But in all of my constructs,
> along with the cytoplasmic expression, I'm also
> getting signal from the nucleus, which was not
> expected. Some people have suggested that EGFP has an
> affinity for DNA that's why in some of the constructs
> we get signal from the nucleus as well. Also some of
> the people here in India, doing experiments with EGFP
> tagged protein, have also observed the migration of
> these proteins into the nucleus when these proteins
> have no role in the nucleus. These observations are
> quite puzzling to me. Could anyone of you please
> through light on this phenomenon.
>
> Thanking you,
> Shagufta Rehman
> PhD student
> All India Institute of Medical Sciences
> New Delhi, India.
>
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Nina Stromgren Allen
Professor of Botany
Director, Cellular and Molecular Imaging Facility
Department of Botany
Box 7612
North Carolina State University
Raleigh, North Carolina 27695-7612
Phone: 919-515-8382, 3525, 2727
Fax:  919-515-3436