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We are thinking of using these dyes as a nuclear stain with a confocal
to contrast with FITC staining on animal cells centrifuged onto
coverslips and paraformaldehyde fxed.

Visualised with an Argon/ion laser

Does anyone have any experience of this fluorochrome?

What about bleaching?

Are there better fluorochromes?
We have tried propidium iodide but it is unsatisfactory due to mRNA
staining.

We only have an Argon ion laser at the moment, HeNe green/red in the
land of grant applications at the moment.



Regards

Leigh Silvester
Division of Animal Physiology
University of Nottingham
School of Biosciences
Sutton Bonington Campus
Loughborough
Leicestershire
LE12 5RD, UK
tel: +44 (0)115 9515151 x18736
fax +44 (0)115 9516302
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