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yes we have - works well
I would image using multitracker if  Zeiss and image the FITC first and 
then the DAPI - this was on a thread many months earlier

Mary Ann wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Has anyone had experience imaging DAPI-stained tissue/cells using a 405 
>laser?  I am told that it works very well even though it appears that only 
>about 6% of the excitation is available at 405nm.  Also, is there a cut-off 
>filter available that would allow double imaging with DAPI (activated by 
>the 405 laser) and FITC (activated by a 488 laser)?  The emission spectra 
>of these dyes overlap, but conceivably, if the DAPI emission could be 
>blocked, perhaps the two could be used together.
>
>Thanks,
>Mary Ann
>
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