>
>sorry, PI stains all DNA and RNA, and has to be used with RNAse to
>highlight nuclei - and it may then well allow one to distinguish between
>normal and apoptotic nuclei.
>

Jonathan,

We have found that PI stains smooth muscle, adventitial and endothelial
cell nuclei with good selectivity (DNA over RNA) in 'fixed' sections of
blood vessels without the use of RNAse.  In our hands it's a great stain
for 'non-viable' cells.

Craig.


______________________________________________________________________________
Craig J. Daly
Research Associate                     tel:       0141 330 6606
CRI Heart Failure                      fax:       0141 337 1651
West Medical Building                  email:     [log in to unmask]
University of Glasgow
Glasgow G12 8QQ.

Web Sites                             http://www.cardiovascular.org
                                      http://www.adrenoceptor.com
______________________________________________________________________________ Ä