> - does anyone know the method for using propidium iodide for
nucleus?
 
 
We have used PI frequently for delineating dead cells in confocal
studies of living tissue (cardiac myocytes) by including PI (about 5
micromol.) in the superfusion buffer. Detect in red channel (exc 568,
em at 640). Remember that in living cells, PI does not cross the
plasma membrane unless the membrane is damaged - I have no
experience with fixed tissue.
 
PI binds to DNA, so the nucleus is almost uniformly stained in
interphase. I don't know if others are looking at nuclear cycles with PI
- might be interesting to hear about this.
 
Ian.
 
 
 
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Dr Ian Harper
Experimental Biology Programme
Medical Research Council
PO Box 19070                         Tel: 027-21-938 0347
Tygerberg 7505                       Fax: 027-21-938 0456
South Africa            Internet: [log in to unmask]
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