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March 2004

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Confocal Microscopy List <[log in to unmask]>
Subject:
calcium indicators
From:
Kathy Spencer <[log in to unmask]>
Date:
Wed, 17 Mar 2004 15:58:44 -0800
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Hi All!
        Having difficulties with ligand autofluorescence in calcium imaging. We
measure an auto-fluorescence peak around 460-570nm emission, and are trying
Fura-2 and Rhod-2 calcium indicators. Our compound still interferes with
the emission of these dyes. Can anyone recommend other calcium dyes we
could try? We found that the Fluo dyes are right in the middle of our
autofluorescence peak, but are there other dyes?
        Thanks!
        Kathy Spencer



Kathy Spencer, Ph.D.
Scientific Associate
The Scripps Research Institute
10550 N. Torrey Pines Road
ICND 202
La Jolla, CA 92037
858-784-8437

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