CONFOCALMICROSCOPY Archives

August 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 20 Aug 1997 09:12:15 +1000
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>Dear BioRad users:
>
>I am recording dual images on a BioRad MRC 600 confocal microscope. The left
>channel is transmitted light with DAB label, the right channel is a FITC
>label. I would like to merge the two images but maintain the transmitted
>image as black and white, not the pseudocolor green that is the default.
>In the 'merge' section of the manual is a brief mention of this ability,
>altering the combination of colors by somehow enabling different color guns.
>
>Does anyone have any idea how to do this? I think the resulting pictures
>should look interesting.
>

In the Contrast menu of COMOS or SOM simply select which of the merged
images you want (by hitting M or clicking on mergetable) and turn on
or off whichever guns you want by typing r, g or b or clicking the
corresponding buttons.

                                                Guy Cox

Dr. Guy Cox,   |                    ooOOOOOOoo
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