In addition to those sources already mentined, you might want to chek the
following:

BA Pollok and R Heim, "using GFP in FRET-based applications, 1999. Trends Cell
Biol 9:57-60

Ellenberg J, et al.
     Dual-colour imaging with GFP variants.
     Trends Cell Biol. 1999 Feb;9(2):52-6.

Ellenberg J, et al.
     Two-color green fluorescent protein time-lapse imaging.
     Biotechniques. 1998 Nov;25(5):838-42, 844-6.

Hope this helps,

Steffen


--- Sue DeMaggio <[log in to unmask]> schrieb:
> Hello List - I have 'serv' you can do for me....Please excuse me if this is
> a crazy question, but I haven't done FRET before and we have a question -
> actually 3 questions.  1.) Is BlueFP different from CyanFP?   2.) Does
> anyone have the corresponding excitation and emission wavelengths for
> BFP-GFP vs CFP-YFP and 3.) have you done them on a confocal?  Can't seem to
> find any confocal references!   Thanks
> Sue
> Susan DeMaggio, MS BSMT(ASCP)QCym
> Resource Manager
> Optical Biology Shared Resource
> University of California, Irvine
> Irvine, CA  92697-2275
> phone   949-824-4110
> fax     949-824-3571
>
> http://calweb.bio.uci.edu/OBC/index.htm
>

===
Dr. Steffen Dietzel
Dept. of Cell and Structural Biology
University of Illinois at Urbana Champaign
601 S. Goodwin Ave.
Urbana, IL 61801
Phone: +1/217/333-8372  Fax: +1/217/244-1648
e-mail: [log in to unmask] Web:  http://www.life.uiuc.edu/belmont/dietzel
__________________________________________________________________
Do You Yahoo!?
Gesendet von Yahoo! Mail - http://mail.yahoo.de
Yahoo! Auktionen - gleich ausprobieren - http://auktionen.yahoo.de