Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I have used a complicated but neat and effective sealant which also
works well with live material.
Use Valap, a mix of vaseline, lanolin and parafin, 1:1:1.  To use,
heat gently until melted.  Then bend a paper clip into an L-shape and
heat the bent end in a bunsen burner.  Dip the corner into the valap.
The drop that forms will stay liquid long enough to apply drops at
the corners or a streak along the side of the cover slip.  With a
little practice you can make very neat slides.  I don't know how long
they last, as I only worked with live material.

Carol

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>I've heard varying opinions regarding the use of sealants for slides
>mounted in fluorescence friendly media such as Vectashield.  We are
>looking at eGFP cells with immuno label for cell type markers visualize
>with either Cy3 or Cy5.  Is it true that nail polish is problematic for
>eGFP preps and if rubber cement is a better sealing option, any
>suggestions on how to apply it best? We've tried applicator sticks or
>pastuer pipets with more mess than nail polish.  Any secrets that can be
>shared?
>
>Thanks!
>Mary Ellen
>
>Mary Ellen Pease, M.S.
>Laboratory Manager
>Glaucoma Research Lab & Microscopy and Imaging Core Facility
>Wilmer Eye Institute, Johns Hopkins Hospital
>175 Woods Research
>600 N. Wolfe Street
>Baltimore, MD 21287
>410-955-3337 (phone/voicemail)
>443-287-2711 (fax)
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>
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Carol Bayles
Microscopy,  Imaging & Fluorimetry (MIF)
Biotechnology Resource Center
160a Biotech Bldg
607-254-4860
www.brc.cornell.edu

Confocal and Multiphoton Microscopy
Nanobiotechnology Center
www.nbtc.cornell.edu

Cornell University
Ithaca NY 14853