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Hi Li,
I've seen good results with Hoechst 33258.  This requires UV illumination.
If you have a CCD camera or photomultiplier tube, then DRAQ5 would probably
work.  It emits in the far red so you need a device to "see" it.  Both of
these dyes may alter normal cell proliferation.
Good luck,
Carl


Carl A. Boswell
Dept. of Mol. Cell. Biology
Univ. of Arizona
(520) 626-8469
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----- Original Message -----
From: "Yue Li" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Tuesday, May 14, 2002 11:13 AM
Subject: Vital labeling for nuclei


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Greetings,
>
> Sorry for the confusion in the last email. I'm actually looking for
something
> that can vitally label the neclei so that I can observe the cells under in
> vivo microscope. Any information is highly appreciated.
>
> Yue Li
> Univ. of TX at Austin