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Date: | Wed, 1 Oct 2003 15:22:04 +0200 |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Becker and Hickl is selling hardware that can be connected to many
microscope systems which does support FCS measurements, but only in
combination with a pulsed (multi-photon) laser. We use the Zeiss FCS
system which after some stratup problems seems to work fine. We are in
the process of comparing results with FRAP results (diffusion times). We
are mainly interested in proteins in cells and this is not so very easy
and straith forward. However the very sensitive detector works great
with low levels of proteins, like proteins with native promotors or
transgenic GFP mice cells.
Regards, Gert
Ian Gibbins wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello Confocalists
>
>
> We have some users who are keen to set up to do fluorescence correlation
> spectroscopy (FCS), which is a technique I know little about, although I
> know in principle what is involved.
>
> From what I can gather, the Zeiss FCS system, based on their LSM 510 is the
> only commercial confocal that can do this. Is this true? Do any users out
> there have experience with the Zeiss (or any other) FCS system?
>
> Reply off-list, if you prefer. I'd also be interested in hearing from other
> manufacturers, if appropriate.
>
> Thanks
>
> IAN
>
>
>
>
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