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Dear all,
I am using a CSU-22 spinning disc for calcium imaging in dendrites and 
spines of neuronal cells. When doing synaptic stimulation, the glass 
pipette is positioned pretty close to the imaged region and therefore 
reflects the excitation light. My question: does anybody have 
experience with non-reflecting coatings that can be applied to glass 
pipettes and are of no effect on the tissue? We thought about black 
nail polish and would like to know if anybody has a betteer suggestion.
Thanks a lot,

Friedrich


Dr. Friedrich W. Johenning
NWFZ -Campus Mitte / AG Schmitz
Charite University Medicine Berlin
Charitéplatz 1
10117 Berlin
Tel.: 030 450 528077
Mobil: 0173 2144701
Fax: 030 450 539943