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we have excited Alexa 568 with the 488 line, works 'perfect' (I don't know
about the other Alexas). I wondered if it could work, before, just tried
it, we could get +/- the same pics as with the 543 HeNe (set to 100% power)
line, which is of course relatively week (Leica SP1).
Arthur


At 17:06 28.02.2004, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi friends,
>in experiencing FRET with Alexa555, Alexa647 and Alexa 488 we had a lot
>of problems since we have a compartively high signal when we excite
>Alexa555 and Alexa647 at 488.
>Did anyone of you experience the same problem?
>We also implemented a good alghoritm published by Ammasi Perisamy's
>group on Biophysical journal last year but now we are looking for more
>secure fluorescent molecules to be used for FRET experiments.
>Suggestions are welcome.
>Alby
>
>
>........................................................................
>....................................
>... non basta, resistere, resistere...resistere
>^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>Alberto Diaspro
>Department of Physics, University of Genoa
>Via Dodecaneso 33, 16146 Genoa, Italy
>voice: +39-0103536426/480  fax 010314218
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Dr. Arthur Schuessler
Institute of Botany, FB10, TU Darmstadt
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