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Are you referring to RAM or harddisk space?  As far as I know, LaserSharps 
writes to temporary files rather than holding the experiment in RAM.  We 
have a Radiance 2000 on NT (1 GB RAM, 1.5 GB page file) with LaserSharp 
5.2 build 824.  The collection limit is 2 GB.  You may want to check 
"System Configuration | Scan system" for the setting of the collection 
limit.  Previous versions may have a 1 GB limit.  Also check the location 
of the temporary files which needs to have plenty of space (as much as you 
want to acquire each time or more).  Good luck.

-- 
Pang (Wai Pang Chan, [log in to unmask], PAB A087, 206-685-1519)
The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)

On Fri, 17 Mar 2006, Michael Cammer wrote:

> We have the Radiance 2000.  This is a major limitation of the system.
> We've had to compromise by restarting long time lapse experiments in the 
> middle, by taking fewer Z planes per timepoint or longer intervals.
> But when our BioRad confocals die, LaserSharp software should be obsolete.
> -Michael
>
> At 05:51 PM 03/14/06 -0800, you wrote:
>> 
>> I have a question regarding a BioRad 1024 confocal system.  This system is
>> working fine.  But one trouble is that people cannot run any scanning
>> program (time lapse experiments) that contains more than 230 mb, although
>> the system has plenty of free memory (we just added another 1gb to it).  I
>> will appreciate if anyone can share with us if you had same experience and
>> managed to resolve it.