CONFOCALMICROSCOPY Archives

July 2001

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Re: unstable focus on Axiovert 100
From:
F Javier Diez Guerra <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 26 Jul 2001 17:16:37 +0200
Content-Type:
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Dear Listmembers,

We are currently doing time-lapse series during 2-4h periods using Axiovert
100 and Axiovert 135 estatives and have the same problems. The focus drift
is of such magnitude that it forces us to be constantly (every 4-5 min)
adjusting the micrometric control manually. One of the micros is set on a
high-inertia table and we are using closed perfusion chambers with its own
temp control.

It would be of great help to know how other colleagues have resolved this
issue.

Best regards,


F Javier Diez-Guerra
Servicio de Microscopia Confocal
Centro de Biologia Molecular Severo Ochoa
Facultad de Ciencias, UAM
28049 Madrid, Spain

Tel     34-913978051
Fax     34-913978087

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