Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We have viewed GFP in skin as distinct from autofluorescence with the laser
tuned up to 890 nm.

At 11:13 PM 12/8/2003 -0500, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>We are trying to visualize the intradermal behavior of EGFP-expressing
>Leishmania parasites in mouse ears. A hurdle is the high background
>autofluorescence from keratinocytes, hairs, and hair follicles. Given that
>our samples were excited with a 2-photon laser at 800 nm, should I
>consider to tune the laser to a longer wavelength? Any additional
>suggestions on potential ways to reduce the autofluorescence are very much
>appreciated.
>
>Hai Qi, M.D., Ph.D.
>
>Postdoctoral Fellow
>
>Laboratory of Immunology, NIAID
>
>National Institute of Health
>
>Bldg. 10, Rm 11N250
>
>10 Center Dr., MSC 1892
>
>Bethesda, MD 20892
>
>
>
>TEL: 301-4961979
>
>FAX: 301-4807352
>
>EMAIL: [log in to unmask]

____________________________________________________________________________
Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of Med.
Jack & Pearl Resnick Campus      1300 Morris Park Ave.     Bronx, NY  10461
(718) 430-2890       Fax:  430-8996      URL:  http://www.aecom.yu.edu/aif/