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I suffered exactly the same problem in pancreatic acinar cells, even
lowering power of excitation light. I think to take an image before
and after treatment, at given times, could avoid bleaching.
Good luck and let us know your progress!
Dr Pedro J Camello
Dept Physiology
Fac Veterinary Sci
Univ of Extremadura
Campus Universitario
10071 Caceres
SPAIN
Phone/Fax: 927-25-71-54
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