We have used the slide set made by applied precision to check filed
illumination. the data is described in the Cytometry paper. In our opinion
it is much better to use these plastic fluorescent slides than a
suspension of fluorescein in a well slide
bob
Robert M. Zucker, PhD
U.S. Environmental Protection Agency
MD 72
National Health and Environmental Effects Research Laboratory
Research Triangle Park, North Carolina, 27711
Tel: 919-541-1585; fax 919-541-4017
e-mail: [log in to unmask]
"Donnelly, Tom"
<[log in to unmask]> To: [log in to unmask]
Sent by: Confocal cc:
Microscopy List Subject: Re: Confocal QA or Pretty Pictures
<[log in to unmask]
FFALO.EDU>
10/02/01 12:55 PM
Please respond to Confocal
Microscopy List
Barbara:
We supply a three slide set similar to what you describe below for
illumination calibration.
One slide for the lower DAPI like probes, one for the FITC,Rhod, wavelength
probes, and another for the higher wavelength probes including Cy5.
If you are interested, you can contact: [log in to unmask]
Set of Fluorescent Calibration Slides 52-503256-003 $60.00
Regards,
Tom
Tom Donnelly Applied Precision, Inc.
Biotechnology Group 1040 12th Ave. N.W.
(425)557-1055 fax Issaquah, WA 98027-8929
[log in to unmask] http://www.api.com/products/bio/deltavision.html
-----Original Message-----
From: Barbara Foster [mailto:[log in to unmask]]
Sent: Tuesday, October 02, 2001 12:57 PM
To: [log in to unmask]
Subject: Re: Confocal QA or Pretty Pictures
Hey, Ray,
MME is in the process of putting together just such a slide set:
4 slides which have spectra similar to FITC, Rhodamine/Texas Red, Acridine
orange, and DAPI.
Is that the sort of thing you need?
Best regards,
Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suite 102
Springfield, MA 01118
PH: 413-746-6931 FX: 413-746-9311 Web: www.MME-Microscopy.com/education
"Why didn't they teach us that sooner?" ... probably because no one
thought to call MME about customized, on-site courses. Offered in all
areas of microscopy, sample prep,and image analysis, they make an immediate
impact on your productivity.
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At 10:24 AM 10/2/01 -0500, ray hester wrote:
>Ten years or so ago when we purchased our first CLSM, an ACAS 570,
Meridian
>Instruments, the manufacturer, supplied us with a plastic slide
impregnated
>with a 'fluorescent' compound that could be used to monitor your system's
>performance on a day-to-day basis. You set the laser power, PMT gain,
etc.,
>to the same settings each day, focused the same distance into the slide,
and
>clicked the space bar and it provided a graph of fluorescence vs AOM
setting
>(from 0 to 100) which could be tracked.
>
>Even something as primitive as a fluorescent slide might be a help with
our
>new confocal, although certainly we/they are capable of a more
sophisticated
>system today.
>
>Ray Hester
>Univ. of South Alabama
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