CONFOCALMICROSCOPY Archives

June 2010

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Michal Opas <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 4 Jun 2010 17:49:55 -0400
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Well, the List is my last hope.
The problem:
after refurbishing Kr/Ar laser and re-installing it I am not able to get 
a sharp image on the monitor. My gut feeling is that it probably is not 
related to laser but a problem with a scan head. So laser outputs fine, 
staining (as excited by Hg burner) looks spectacular but to get an 
output pinhole has to be open and gain cranked up. ND filter is on 1. 
And the image still look blurry.
Any advice as to how to approach the problem shall be greatly appreciated.

Michal

-- 

*9^th International Calreticulin Workshop will be held in 
**Copenhagen**, **Denmark** on **August 29^th -31^st 2011**. For 
additional information please visit:** http://www.crt2011.com/*


* Dr. Michal Opas
      Professor
      Department of Laboratory Medicine and Pathobiology
      University of Toronto
      1 King's College Circle
      Medical Sciences Building, room 6326
      Toronto, Ontario, M5S 1A8 Canada

**°°°°°°°°°°°°°**
  phone: (416) 978-8947 (laboratory)
         (416) 971-2140 (office)
    fax: (416) 978-5959
e*-*mail: **[log in to unmask] <mailto:[log in to unmask]>***

*WWW**:** **http://www.utoronto.ca/mocell
<http://www.utoronto.ca/mocell>*



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