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Hello Julia
We've found AMCA to be much better than Cascade Blue in wide-field
fluorescence: brighter, less fading, less emission overlap with
fluorescein-like fluorophores, and a lot of secondary antibodies are
available conjugated to it. I haven't tried it in confocal imaging
since we do not yet have an appropriate laser line.
hope that helps a bit
IAN
On Saturday, November 5, 2005, at 09:19 PM, Julia Edgar wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear All
> Would you please recommend a good blue fluorophore. Currently I'm
> using cascade
> blue but it bleaches very quickly compared to e.g. FITC or rhodamine.
> Thank you in advance
>
> Julia Edgar
>
>
>
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Prof Ian Gibbins
Anatomy & Histology
Flinders University
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