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Thank you Ian
Julia
Hello Julia,

AMCA is probably the most widely available blue (~365 nm ex/~440 nm em)
dye for conjugates.  There is also AlexaFluor 350 which is pretty much
equivalent to AMCA spectrally but can be a little brighter depending on
conditions.

Other options around this general range are Pacific Blue, Marina Blue
and AlexaFluor 405.

Ian Clements
Molecular Probes Labeling and Detection Technologies.
Invitrogen Corp


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Julia Edgar
Sent: Saturday, November 05, 2005 2:49 AM
To: [log in to unmask]
Subject: Blue fluorophore

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Dear All
Would you please recommend a good blue fluorophore. Currently I'm using
cascade
blue but it bleaches very quickly compared to e.g. FITC or rhodamine.
Thank you in advance

Julia Edgar



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