Dear Susan,

which company produce ToPro.

Some of you can suggest me the best working concentration?

Thank you

Cristiano

At 14.33 30/10/01 -0500, you wrote:
>Rich,
>
>We've used ToPro and it works very well.  Just remember that you will have
>to use RNAse also since ToPro stains both RNA and DNA.
>
>Susan Garfield
>Manager, Confocal Microscopy Core
>Laboratory of Experimental Carcinogenesis
>Center for Cancer Research
>National Cancer Institute, NIH
>
>-----Original Message-----
>From: richard green [mailto:[log in to unmask]]
>Sent: Wednesday, October 24, 2001 6:49 PM
>To: [log in to unmask]
>Subject: Nuclear stains
>
>
>Howdy,
>On the Confocal, I am trying to stain Nuclei in
>Cultured Tumor cells. What would you all suggest would
>be the best nuclear stain (in the 633nm range), would
>it be ToPro? I may be using Alexa 488 or 568 as a
>secondary. Any advice would be appreciated. Thanks
>
>
>-Rich
>
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Prof. Cristiano Rumio
Department oj Human Anatomy
Via Mangiagalli 31
20133 Milan
Italy
Tel. +39-0258355401
Fax. +39-022364082
e-mail [log in to unmask]