Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

see
http://www.aecom.yu.edu/aif/instructions/fluorescence/probes_gfp_dsred.htm

dsRed has a large green component.

switch to a monomeric red protein.

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Have you tried scanning sequentially rather than simultaneously? The
> Leica has a great protocol for doing this.
>
> Judy
>
>
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 7Queen
> 30 Bond St.
> Toronto, ON M5B 1W8, Canada
> ph:  416-864-6060  x6337
> pager: 416-685-9219
> fax: 416-864-6043
> [log in to unmask]
>
>>>> Joel Sheffield <[log in to unmask]> 05/11/06 3:35 PM >>>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Greetings,
>
> I have been working with someone who is attempting to do double label
> studies using DSRed2-mito and an FITC-based antibody system.  We
> disscovered, using the spectral scan of the Leica SP system, that
> there is significant fluorescence of the DSRed in both the green and
> red wavelengths when we illuminate with a 488 argon line.  Indeed,
> the spectrum appears to have two peaks, of almost equal intensity, in
> response to this excitation.  As a result, we can not separate the
> DsRed Signal from the FITC when the FITC is weak.
>
> At the risk of sounding like a complete novice, has anyone worked out
> a way to deal with this problem?
>
> Joel
>
>
> Joel B. Sheffield, Ph.D.
> Biology Department, Temple University
> 1900 North 12th Street
> Philadelphia, PA 19122
> [log in to unmask]
> (215) 204 8839, fax (215) 204 0486
> http://astro.temple.edu/~jbs
>


_________________________________________
Michael Cammer
Analytical Imaging Facility and
Dept. ASB Biophotonics Innovation Laboratory
Albert Einstein College of Medicine
1300 Morris Park Avenue, Bronx, NY  10461
718-430-2890  Fax 718-430-8996
work:  http://www.aecom.yu.edu/aif/
personal:  http://coxcammer.com/