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I hate to do this, Clare, but I am going to disagree with you.  I  
really like the Bioptechs system.  TEmperature uniformity and  
stability are excellent.  More importantly, I like the heated lid  
setup for long term culture without evaporation or the need to  
perfuse.  I have not seen a comparable lid system for Warner, so if  
it exists I would be interested to hear about it.  I have not used  
the perfusion system on Bioptechs so can't comment if that is what  
you need. We reuse the Bioptechs dishes until the bottoms fall out,  
so they are more expensive than coverslips, but not as bad as you  
might think from first look.  If just looking at cells in media for  
long term, it works great.  Dave

Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)


On May 25, 2006, at 1:25 PM, simon watkins wrote:

> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi- 
> bin/wa?S1=confocal
> We use two types of chambers.  The Bioptechs FCS2 for long term  
> expts (easy fluid exchange and sealed to keep out bugs), for short  
> term expts we use the Harvard PDMI2 with Mattek dishes.  It’s a  
> doddle to use, but more importantly wrt to alisons question you can  
> warm or cool as you wish (well certainly between 18-37C).  NB. Big  
> temperature changes means big focus changes so you need to have one  
> of the nifty ASI/Nikon/Olympus autofocus gizmos as well if you want  
> to work at high resolution.
>
> Simon
>
> Simon C. Watkins Ph.D. FRC Path.
> Professor, Cell Biology and Physiology and Immunology
> Director Graduate Program in Cell Biology and Physiology
> Vice Chair, Cell Biology and Physiology
> Director Center for Biologic Imaging
> BSTS 225
> University of Pittsburgh
> 3500 Terrace St.
> Pittsburgh PA 15261
> Tel:412-648-3051
> Fax:412-648-2797
> URL: http://www.cbi.pitt.edu
> From: Confocal Microscopy List  
> [mailto:[log in to unmask]] On Behalf Of Alison North
> Sent: Thursday, May 25, 2006 1:17 PM
> To: [log in to unmask]
> Subject: Re: Temperature Control - what about cooling?
>
>
> Hi all,
>
> I'm totally with Clare re the Warner chambers - I love the  
> flexibility too.  But I have a different question - we have indeed  
> had lots  of discussions about heating chambers, but what about  
> cooling ones?  I have many users working on flies, worms, yeast and  
> trypanosomes - and they would be like to be able to cool their  
> samples, at least to say 18 degrees, and then switch the  
> temperature rapidly.  Problem is, many of the cooling systems seem  
> to be rather big and clumsy with tubes coming out all over, which  
> won't work on some of our microscopes.  So I am considering the  
> Linkam stages, and I just wondered whether anybody had any  
> experience with these?  (or any better suggestions?)
>
> Please feel free to contact me off-list if you'd prefer.
>
> Thanks in advance for any comments!
> Alison
>
> Dr. Alison North
> [log in to unmask]
>
>
> Clare Waterman wrote:
>
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> I like the warner because of so many options – one controller, many  
> options-  inline fluid heaters, objective heaters, specimen  
> heaters, all work with normal coverslips-  also good prices.  Very  
> flexible in terms of mixing and matching components for specific  
> needs (perfusion, open chambers closed chambers, etc.)
>
>
>