CONFOCALMICROSCOPY Archives

January 2004

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show HTML Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
antibody as a blocking reagent
From:
Louis Villeneuve <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 13 Jan 2004 10:56:58 -0500
Content-Type:
multipart/alternative
Parts/Attachments:
text/plain (552 bytes) , text/html (1404 bytes) 
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi everyone,

One of my "customers" here wants to look for inhibition of a specific protein by incubation with an antibody in living cells.

 What would be the diluent of choice for the inhibition solution ( with the antibody)?

We have tried once and the "inhibition" antibody was diluted in a physiological buffer.  We got a lot of non-specific staining.  I should use a carrier protein with the buffer to dilute the "inhibition" antibody, but wich one?

thanks!

Louis

ATOM RSS1 RSS2