Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Michael, Numerical data in Excel for Rhodamine Green and lots of fluorescein entries (sorry, no flourescein - is this used in green bread?) are at http://home.earthlink.net/~pubspectra/ <http://home.earthlink.net/~pubspectra/> Use the index file (second worksheet tab) at http://home.earthlink.net/~lightmicroscopy/McNamara_Boswell_000_INDEX_Spectr a.xls <http://home.earthlink.net/~lightmicroscopy/McNamara_Boswell_000_INDEX_Spect ra.xls> to find the file and column. The dye data are in http://home.earthlink.net/~gfpology/McNamara_Boswell_Spectra_Dyes_FPs.zip <http://home.earthlink.net/~gfpology/McNamara_Boswell_Spectra_Dyes_FPs.zip> For those interested in filters, lamps, etc, see http://home.earthlink.net/~lightmicroscopy/McNamara_Boswell_Spectra_Filters_ Lamps.zip <http://home.earthlink.net/~lightmicroscopy/McNamara_Boswell_Spectra_Filters _Lamps.zip> The current Invitrogen/MPI spectra sites and those from BD, Zeiss/Bio-Rad, and Carl Boswell & my spectra graphing web sites are listed further down the PubSpectra page. I'm currently gearing up to add more filter sets, some objective lens transmission curves (finally!), and some multiphoton excitation spectra. I strongly encourage everyone who publishes spectra to include numerical data as supplemental online material and/or include in the paper a web link to your website and post the data there. Best wishes, George -----Original Message----- From: Michael Cammer [mailto:[log in to unmask]] Sent: Monday, November 07, 2005 6:29 PM To: [log in to unmask] Subject: spectra Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal It appears that Invitrogen has killed the Molecular Probes web site resource. Does anybody know how to find it within the Invitrogen web site or where else I could find the spectra of many of the dyes? Specifically, right now I need to compare Rhodamine Green to Flourescein. Any help appreciated. Thanks. _________________________________________ Michael Cammer Analytical Imaging Facility and Dept. ASB Biophotonics Innovation Laboratory Albert Einstein College of Medicine 1300 Morris Park Avenue, Bronx, NY 10461 718-430-2890 Fax 718-430-8996 work: http://www.aecom.yu.edu/aif/ personal: http://coxcammer.com/