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Hi Tom,
We have a Leica SP2, it was installed in August, and we have had very few
problems with it. We have had a few problems with the z-stage, but Leica
usually gets a service rep out within a few days. The scan head is fiber
coupled to the lasers, which means a couple of things: it lets you switch it
between different stands (an upright and an inverted), but it also means that
laser intensity is drastically reduced. This isn't a problem for imaging, but
it does take a little while to do photobleaching, and probably won't have
enough power to do uncaging experiments (we don't have a UV laser, so this is
pure speculation). On the plus side of things, the system is very user
friendly, and the software is almost point and click (it usually only takes new
users a couple of hours to get good at using the scope). The prism-based
emission detection system is much easier to use than filter based systems, and
allows you to fine tune the separation of emissions. In general I'm very happy
with the system and would reccomend it. If you have any specific questions,
I'd be happy to answer them.
Cheers,
Russell
--
Russell McConnell
Confocal Imaging Facility Technician
Tufts University School of Medicine
Dept. of Neuroscience
M&V Building room 137
phone: 617-636-3795
http://www.neurosci.tufts.edu/Imaging
Quoting Tom Gore <[log in to unmask]>:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> We are looking for thoughts from list members about purchasing a
> modern
> LSMs, especially from Nikon and Leica. We would be very interested in
> comments from users who have recently bought one. User-friendliness,
> ease
> of operation and need for little maintenance will be major factors
> that
> will influence the final decision.
>
>
> _____________________________________
> Tom Gore, Advanced Imaging Laboratory
> Biology Department, University of Victoria
> Box 3020, Station CSC,
> Victoria, BC, V8W 3N5 Canada
> voice (250) 721-7134 fax (250) 721-7120
> web: http://web.uvic.ca/ail/
>
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