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Hi Andrew,

does your CCD camera attached to the side or bottom port of the scope?

Which scope?

Which filters - Chroma HQ series or Semrock's sputter coated?

Likely it is a bleed of the LED light in your scope.

I have seen it with Chroma non-ET filters from the bottom port of the Nikon 
TE2000E2 scope. Now Nikon has a solution to the problem.

Vitaly
NCI-Frederick,
301-846-6575

----- Original Message ----- 
From: "Andrew Resnick" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Friday, June 15, 2007 3:00 PM
Subject: narcissism?


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> All,
>
> This isn't a confocal question per se, but has anyone else had this 
> problem (narcissism)? Sometimes our images have a large L-shaped ghost 
> which I believe to be the CCD array seeing a reflection of itself.  This 
> usually happens during low-light level imaging (mostly fluorescence 
> methods) and has an intensity around background levels.  Any hints or tips 
> would be appreciated.
>
> Andy
>
> Andrew Resnick, Ph. D.
> Instructor
> Department of Physiology and Biophysics
> Case Western Reserve University
> 216-368-6899 (V)
> 216-368-4223 (F)