On Tue, 13 Feb 1996 09:32:47 +0000 Sabine Fischer wrote:
>
> Ellen Lumpkin wrote that she has a protocol for estimating axial
> resolution for the BioRad confocal microscopy.
> I use an MRC 600 and I am very interessted in this protocol.
> I also would like to know if anybody has measured axial resulution
> for an MRC 600.
>
>
Dear Sabine,
With 0.1 um beads on Jon Lederer's microscope there was a reolution of
0.4 um (x-y) by 0.8 (z) with a Neofuor 63x 1.25 objective. This figure
is given in Cannell, Cheng & Lederer (Spatial non-uniformities in
Ca2+i during excitation contraction coupling in cardiac myocytes.)
Biophys.J 67, 1942-1956. 1994
Regards Mark Cannell
[log in to unmask]