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We use two types of chambers. The Bioptechs FCS2 for long term expts (easy
fluid exchange and sealed to keep out bugs), for short term expts we use the
Harvard PDMI2 with Mattek dishes. It's a doddle to use, but more
importantly wrt to alisons question you can warm or cool as you wish (well
certainly between 18-37C). NB. Big temperature changes means big focus
changes so you need to have one of the nifty ASI/Nikon/Olympus autofocus
gizmos as well if you want to work at high resolution.
Simon
Simon C. Watkins Ph.D. FRC Path.
Professor, Cell Biology and Physiology and Immunology
Director Graduate Program in Cell Biology and Physiology
Vice Chair, Cell Biology and Physiology
Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St.
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-2797
URL: <http://www.cbi.pitt.edu/> http://www.cbi.pitt.edu
_____
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Alison North
Sent: Thursday, May 25, 2006 1:17 PM
To: [log in to unmask]
Subject: Re: Temperature Control - what about cooling?
Hi all,
I'm totally with Clare re the Warner chambers - I love the flexibility too.
But I have a different question - we have indeed had lots of discussions
about heating chambers, but what about cooling ones? I have many users
working on flies, worms, yeast and trypanosomes - and they would be like to
be able to cool their samples, at least to say 18 degrees, and then switch
the temperature rapidly. Problem is, many of the cooling systems seem to be
rather big and clumsy with tubes coming out all over, which won't work on
some of our microscopes. So I am considering the Linkam stages, and I just
wondered whether anybody had any experience with these? (or any better
suggestions?)
Please feel free to contact me off-list if you'd prefer.
Thanks in advance for any comments!
Alison
Dr. Alison North
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Clare Waterman wrote:
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I like the warner because of so many options - one controller, many options-
inline fluid heaters, objective heaters, specimen heaters, all work with
normal coverslips- also good prices. Very flexible in terms of mixing and
matching components for specific needs (perfusion, open chambers closed
chambers, etc.)
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