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Dear All
I have been following this thread.
I am a bit lost, mind you a barely have time to sleep anymore.
We have a Zeiss Axioskop2 with a Pascal head.
I did not experience the same problem (so far).
I remember vaguely that in the maintenance icon under
lenses you had to set the focal position of each lens.
(done by Anaspec for us!)
Each time you change the lens position the stage drop
before rotating the nose peace.
Just a odd question for your guys out there tat live
on/next to CLSM systems. Is this not related to the amount
the stage drop to get the next lens in place?
Will offsetting the focal position not solve the problem?
(not the ideal but might it work?)
-----Original Message-----
From: Glen MacDonald [mailto:[log in to unmask]]
Sent: Wednesday, July 30, 2003 6:45 PM
To: [log in to unmask]
Subject: Re: Warning: potential lens damage with Zeiss Axiovert 200 and
Zeiss motorized scanning stage
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Dear Xuejun,
The potential to damage a lens is not limited to Zeiss motorized
stages. The ASI motorized stage on our Axiovert 200 will also smack
the objectives adjacent to the currently used lens. Also, even if the
stage is merely close to, but not touching, the adjacent lenses,
hitting a button to rotate lenses can scrape the end of a lens against
the lower edge of the sample carrier. The 40X/.75 NA PlanNeofluor
projects slightly higher than the 63X Planapo.
At least with the ASI stage the travel limits in X and Y are easily
set, so we haven't damaged a lens, yet.
Regards,
Glen
On Friday, July 25, 2003, at 01:19 PM, xjsun wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello everyone,
>
> After 2 lenses damaged, we finally find out how it happened. This
> applies to specific combination of Zeiss Axiovert 200 and Zeiss
> motorized scanning stage.
>
> When the stage is moved to far left (or right) position, a supporting
> frame under stage will hit the objective (high NA) next to the
> currently
> used lens. The final result is that there will be a dent right in the
> middle of the objective. This will lead to broken seal of the lens.
>
> What a wonderful piece of engineering Zeiss produced! Of course,
> repairing the lens costs a fortunate and takes forever (6 months now).
>
> Raising the stage will reduce the chances but does not solve the
> problem.
>
> Hope this would not happen to anyone else and I am also curious to know
> if anyone has lens damaged in similar way with such combination of
> scope
> and stage.
>
> Best wishes,
>
> Xuejun
>
> --
> Xuejun Sun, Ph.D.
> Dept. Exp. Oncology
> Cross Cancer Institute
> 11560 University ave.
> Edmonton Alberta T6G 1Z2
> Canada
>
> Phone: (780) 432-8898 (office)
> (780) 432-8468 (lab.)
> Fax: (780) 432-8425
>
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
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