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November 2006

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Confocal Microscopy List <[log in to unmask]>
Subject:
Autofluorescence in TISSUEFIX preserved tissue
From:
Louis Villeneuve <[log in to unmask]>
Date:
Mon, 20 Nov 2006 08:54:32 -0500
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Hi everyone,

We have open aorta (mouse, 2-3mm in diameter) preserved (immerged, 4C) in 
TISSUEFIX.  We want to do some indirect immunoflurescence on it.  2 
questions:

1- How does TISSUEFIX  fix the tissue, cross-linking?
2- What would be the correct procedure to get maximum results in order to 
do immunofluorescence studies.


Louis

Louis Villeneuve
Research Associate- Confocal Microscopy
Heart Montreal Institute- Research Center
5000 East Belanger
Montreal (Qc), Canada
H1T 1C8

514-376-3330 ext 3511
514-376-1355 (Fax)

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