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Dear all

A colleague of ours has asked for  some advice. He has some temporal
bones (including cochlea and the vestibular apparatus), which have stem
cells marked with GFP. The specimens have been fixed in PFA and have
subsequently been demineralised in EDTA. Instead of cryostat sectioning
would it be possible to embed in plastic(Epon) or paraffin or is the
dehydration process incompatible with the GFP? He has not previously
done any work with GFP and the specimens are rather unique so he does
not want to set up a new and untried protocol.

If anyone has any advice, please let me know.

Thanks

Michelle
Dr Michelle Peckham
School of Biomedical Sciences
Worsley Building
University of Leeds
Leeds
LS2 9JT
UK

telephone: 0113 343 4348
fax:       0113 343 4228 
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