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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Nathalia,
Not knowing anything about your setup or what you are looking at, my
first guess would be temperature fluctuations. Try and let the
microscope and stage warm up for a couple of hours, enclose as much
as possible in some sort of chamber (a large bag may work in a pinch).
One check- during z-drift, does the z-encoder indicate that the
z-position is fixed? Another check- try setting upper and lower
limits for a z-stack and (rapidly) move between those tow
setpoints. Is there hysteresis present? Those can tell you if there
is a hardware problem.
Andy
At 08:58 AM 11/8/2007, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi all,
>I need some help. We have a 1 year old Leica SP5 inverted scope and pretty
>much all of a sudden we started to get z-drift. Every time you do a time
>lapse, no matter what the z-step or time interval, the system does not start
>at the top of the sample, it moves down significantly and progressively.
>Anything I can do to fix this?
>
>I have called the service rep but would like to fix the problem ASAP. It is
>possible that I am the first person to use the system for this application
>since we upgraded the software and firmware.
>
>Nathalia
>
>--
>Nathalia Glickman Holtzman Ph.D.
>Department of Biology SB D328
>Queens College, CUNY
>65-30 Kissena Boulevard
>Flushing, NY 11367
>
>Office: 718-997-3678
>Lab: 718-997-3517
>Fax: 718-997-3445
Andrew Resnick, Ph. D.
Instructor
Department of Physiology and Biophysics
Case Western Reserve University
216-368-6899 (V)
216-368-4223 (F)
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