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We have been looking at TIRF systems and recently tested several
white light TIRF systems. Has anyone directly compared laser vs.
white light TIRF? The floating/attached bead demo works fine so it
looks like TIRF. However, when we focus up from the coverslip with
GFP labelled cells, we can see significant fluorescence in focal
planes that should not be getting excited (5 microns up). Is this
white light vs. laser TIRF, or is it that cells present a problem not
seen with beads. Dave
Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)