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We have been looking at TIRF systems and recently tested several  
white light TIRF systems. Has anyone directly compared laser vs.  
white light TIRF?  The floating/attached bead demo works fine so it  
looks like TIRF.  However, when we focus up from the coverslip with  
GFP labelled cells, we can see significant fluorescence in focal  
planes that should not be getting excited (5 microns up).  Is this  
white light vs. laser TIRF, or is it that cells present a problem not  
seen with beads.  Dave

Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)