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We have a Coolsnap FX mono behind a Sutter 
lambda-10 emission filter wheel, that is  used for epifluorescence microscopy.

 From time to time I check illumination flatness, 
alignments, etc. However, when I look at 
transmission samples using brightfield or 
phase-contrast I notice small particles of dust 
located always at same position of the image.

I have examined the microscope and the dust seems 
to be located on the glass in front of the CCD of 
the camera. Careful cleaning with anti-static 
clothe and air-stream only changes the distribution of the dust particles.

After repeating several times the same operation, 
I have realized that the dust comes from small 
particles released in the process of screwing the 
camera into the filter wheel thread.

Has anybody experienced something similar? Any 
recommendation to keep clean the CCD window?

Thanks,


F Javier Diez-Guerra, PhD
Profesor Titular
Centro de Biologia Molecular Severo Ochoa
Facultad de Ciencias, Universidad Autónoma
Ctra Colmenar Viejo Km 15
Cantoblanco, 28049 Madrid
SPAIN

phone:  +34 91 4978051
Fax:      +34 91 4978087
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