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The PS Speck microspheres work quite well, they are bright enough for 2 photon excited fluorescence. With 170 nm they are a bit big to be point sources, but I found them to be a good compromise.

Andreas

-----Original Message-----
From: "Cromey, Douglas W - (dcromey)" <[log in to unmask]>
Sent: ‎13/‎11/‎2015 14:42
To: "[log in to unmask]" <[log in to unmask]>
Subject: resolution standard for MP and SHG?

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I am collaborating with a group that is building a multiphoton microscope. Can anyone recommend resolution test samples for 2P and second/third harmonic imaging? Pictures are nice, but we would like to test performance as well.
Doug

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Douglas W. Cromey, M.S. - Associate Scientific Investigator
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