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May 2005

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Wed, 4 May 2005 11:31:31 +1000
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

George,

The version I gave works perfectly well.  So does the version
you gave!  (Provided you reassemble the line in each case).
I guess the brackets in the version I gave are a different
way of expresssing the same thing.  And it's an interesting
article.

                                                  Guy


Mcnamara, George wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Guy,
>
> I think you meant Webb 2004 OPN - Laser research at Oxford 1960s PMT
> superlinearity = Optics Photoni9cs News page 14-17 (May 2003)
>
> http://www.osa-opn.org/view_file.cfm?doc=%24%28%5C%3F%2DJ0%20%20%0A&id=%24%2
> 9%3C%3B%27IP%20%20%0A
> <http://www.osa-opn.org/view_file.cfm?doc=%24%28%5C%3F%2DJ0%20%20%0A&id=%24%
> 29%3C%3B%27IP%20%20%0A>
>
> (note lack of (%5...%24) in the above URL).
>
>
> George
>
>                 -----Original Message-----
>                 From:   Guy Cox [mailto:[log in to unmask]]
>                 Sent:   Monday, May 02, 2005 5:06 PM
>                 To:     [log in to unmask]
>                 Subject:        Re: Is the 0 to 256 dynamic range linear?
>
>                 Search the CONFOCAL archive at
>                 http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>                 Actually PMTs can be pretty linear devices, so long
>                 as one is not operation at very high or low voltages.
>                 There is an effect which boosts the gain slightly
>                 in the midpoint of the range but proper circuitry
>                 can eliminate that.  It's very clearly explained
>                 at the following reference:
>
>                 http://www.osa-opn.org/
>
> view_file.cfm?doc=%24(%5C%3F-J0%20%20%0A&id=%24)%3C%3B'IP%20%20%0A
>
>                 NB this is a long link and will probably have a
>                 line break so you may need to 'reconstruct' it.
>
>                 The paper mentioned is interesting in its own right,
>                 too - it concerns the early days of lasers.
>
>                 Guy
>
>
>                 > Dear Deborah--
>                 >
>                 > I think that the dynamic range can be linear only if one
> is using photon
>                 > counting.  I'm not sure that's even possible on some
> systems.
>                 >
>                 > Good luck!
>                 >
>                 > Martin Wessendorf
>                 >
>                 >>> When the gain and offset of the detectors are correctly
> set and no
>                 >>> averaging is used, is the 0 to 256 dynamic range linear
> on a confocal
>                 >>> image?
>                 >>>
>                 >>> Many thanks!
>                 >>> Deborah Wessels, Ph.D.
>                 >>> W.M. Keck Dynamic Image Analysis Facility
>                 >>> Room 14 BBE
>                 >>> Department of Biological Sciences
>                 >>> University of Iowa
>                 >>> Iowa City, IA 52245
>                 >>>
>                 >>> phone: (319) 335-2883
>                 >>> FAX: (319) 335-2772
>                 >
>                 > --
>                 > Martin Wessendorf, Ph.D.                   office: (612)
> 626-0145
>                 > Assoc Prof, Dept Neuroscience                 lab: (612)
> 624-2991
>                 > University of Minnesota             Preferred FAX: (612)
> 624-8118
>                 > 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612)
> 626-5009
>                 > Minneapolis, MN  55455                E-mail:
> [log in to unmask]
>                 >
>
>
>                 --
>                 Associate Professor Guy Cox
>                 Electron Microscope Unit,
>                 University of Sydney,
>                 NSW 2006, Australia
>
>                 Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
>                 http://www.guycox.net

--
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net

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