Dear Christophe,

Molecular Probes/Invitrogen sells quite a few versions of anti-GFP antibodies.

If you type in anti-GFP in our browser you can see the complete list.  mAbs, polys, direct conjugates in many Alexa Fluor colors, biotinXX, HRP, even chicken IgY (no Fc tail so low backgrounds).  Some optimized for westerns, most for all applications, especially ICC.   All raised to A. victoria FPs.

Regards,

Mike Ignatius

Molecular Probes/Invitrogen/Life Technologies



________________________________
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Christophe Leterrier
Sent: Monday, October 05, 2009 7:10 AM
To: [log in to unmask]
Subject: good anti-GFP antibody for immunocytochemistry

Dear all,

Sorry for this non-confocal question, but I guessed some of you might be able to recommend an anti-GFP antibody for immunocytochemistry. I want to label cultured cells that have been transfected with a GFP fusion protein (to enhance the GFP signal). I routinely use the Rockland anti-RFP for the same protein fused to mCherry, but now I would like to use the GFP fusion and the signal is a bit weak.

Thank you !

Christophe


--
Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
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