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Hello,
FCS and FCCS can be done with the Olympus FV1000, the Zeiss LSM710 or 780
and the Nikon C1 microscope straightforward without any additional
hardware. You do need the softweare for data analyis though. You can also
perform Raster Image Correlation Spectroscopy (RICS also including ICS,
tICS, and STICS) as noted earlier by Nuno as well as the Number and
Molecular Brightness Analysis (N&B) for determining aggregation of
proteins spatially in an image.
For the FCS part I have up loaded a tutorial for the FV1000. Just copy and
paste this link to access the file:
https://webfiles.uci.edu/xythoswfs/webui/_xy-8018478_1-t_DWD3mp7i
We have many tutorial on line if you want to know more at:
http://www.lfd.uci.edu/globals/tutorials/
I hope this helps!
Michelle
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Thanks so much to everyone for the advice - exactly what I was hoping for
> and really helpful.
> Looks like we'll have to save up for some new detectors then!
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Unruh, Jay
> Sent: 19 April 2012 16:09
> To: [log in to unmask]
> Subject: Re: FCS/FCCS advice
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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>
> One more note: AmCyan is a tetramer and Katushka is a dimer. This could
> cause unwanted interactions of your target proteins or disruption of other
> interactions. I would recommend sticking with monomeric tags if possible.
>
> Jay
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Simon Walker
> Sent: Thursday, April 19, 2012 5:40 AM
> To: [log in to unmask]
> Subject: FCS/FCCS advice
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello,
> Apologies for the rather naive questions, but I have no experience with
> this.
> Someone here is keen to try FCS/FCCS but I'm not sure what we need (both
> in terms of hardware and software) to get this up and running. Can the
> FCS/FCCS data be acquired using a standard confocal microscope (we have
> Zeiss 510, Olympus FV1000 and Nikon A1R confocals with standard PMTs)? If
> so, what tools are available for the data analysis? If it is necessary to
> have some kind of special adaptation (different detectors/hardware
> correlation) what is the most cost effective solution for this?
> The person wanting to do these exps has suggested using AmCyan and
> Katushka for FCCS - can anyone forsee any problems with this combo?
> Thanks in advance for any help..
> Simon
> The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT
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>
--
Michelle Digman, Ph.D.
University of California, Irvine
Laboratory for Fluorescence Dynamics
3204 Natural Sciences II
Office:(949) 824-3255
http://www.lfd.uci.edu/
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