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Hi,

Not straightforward FCS but RICS, Enrico Graton has several papers with it in conventional confocals.

http://www.lfd.uci.edu/~gratton/

also this one for adapting a confocal

http://rsi.aip.org/resource/1/rsinak/v76/i3/p033106_s1


On my PhD I've used Confocor1..with PMTs as far as I remember. Of course detection efficienty is critical.


Also, in theory, if you could park the beam and make the image you should be able to do it, I would say. We are know asking Leica to be able to control the galvos for trying with the new hyd detectors. I must say that I'm not an specialist in the subject, I might be simplistic.

Regarding software I've used a commercial package www.sstcenter.com.

I just found this but never test it.
http://research.stowers.org/imagejplugins/

Looks interesting though...its free and I'm cheap,
Nuno Moreno
CEO @ www.cirklo.org
CTO @ www.igc.pt



Regarding software I'm not a specialist but I've used for my PhD thesis 

On Apr 19, 2012, at 11:39 AM, Simon Walker wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Hello,
> Apologies for the rather naive questions, but I have no experience with this.  
> Someone here is keen to try FCS/FCCS but I'm not sure what we need (both 
> in terms of hardware and software) to get this up and running.  Can the 
> FCS/FCCS data be acquired using a standard confocal microscope (we have 
> Zeiss 510, Olympus FV1000 and Nikon A1R confocals with standard PMTs)?  If 
> so, what tools are available for the data analysis?  If it is necessary to have 
> some kind of special adaptation (different detectors/hardware correlation) 
> what is the most cost effective solution for this?
> The person wanting to do these exps has suggested using AmCyan and 
> Katushka for FCCS - can anyone forsee any problems with this combo?
> Thanks in advance for any help..
> Simon