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Hi Ray,

We're using verapamil hydrochloride (Sigma 381195, 50microM) which seems
to block hoechst efflux pretty well in our cells.  

We also use Fumitremorgin C (Sigma F9054)...Sigma says; "Fumitremorgin C
is a specific, selective, and potent inhibitor at micromolar
concentrations of the breast cancer resistant protein (BCRP/ABCG2), an
ABC transporter associated with chemotherapy resistance. FTC, in
combination with mitoxantrone, can be used for the detection of ABCG2
functional activity in several cell lines.".

Good luck

Sam

Sam Wadsworth PhD
Division of Therapeutics & Molecular Medicine
D Floor, South Block,
Queen's Medical Centre,
University Hospital,
Nottingham 
NG7 2UH

Tel: 0115 8231070
Fax: 0115 8231059
email: [log in to unmask]



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Ray Hester
Sent: 07 June 2007 20:15
To: [log in to unmask]
Subject: which form of verapamil?

Search the CONFOCAL archive at
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Can anyone tell us which one of the four forms of verapamil that
Sigma-Aldrich offers (below) we should add to cell cultures, in which we
are using Hoechst 33342 to stain for ploidy/cell cycle, in order to
stop/lessen the efflux of Hoechst that occurs in some cells.

hydrochloride
hydrochloride hydrate
monohydrochloride monohydrate
and, methoxyverapamil hydrochloride

Thanks.

Ray Hester
Univ of South Alabama

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