On Dec 17,  2:17pm, Nihal Altan wrote:
> Subject: Ratio dyes and confocal sections
> Hi,
>
> Do any of you have experience wih doing confocal z-sections on small ( <
> 0.5uM)  organelles in cells labeled wih a ratio dye.
>
>
> Any comments will be very much appreciated.
>
> Thanks
>
> Nihal Altan
> Rockefeller University NYC NY 10021
> (212) 327 8946
> Nihal Altan
> Box 221
> Laboratory of Cellular Biophysics
> Rockefeller University
> 1230 York Avenue
> New York, N.Y. 10021 USA
> 212-327-8946 (voice)
> 212-327-7543 (fax)
> [log in to unmask] (internet)
>-- End of excerpt from Nihal Altan

Nihal,

We just did some work labeling lysomes with acridine orange, and HRP-DAB.
 Either one worked well for distinguishin the lysomes.  The HRp conjugated with
DAB was nice because our experimental conditions produced a flourecent cell
under all wavelengths (488, 568, 647).  The HRP-DAB allowed for nice
reflectance images.  Hope this helps.

--
Jonathan T. Boyd
Fox Chase Cancer Center
7701 Burholme Ave.
Philadelphia, PA 19111
[log in to unmask]